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rat antibody against mbp  (Novus Biologicals)


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    Structured Review

    Novus Biologicals rat antibody against mbp
    Rat Antibody Against Mbp, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+antibody+against+mbp/MBP+Antibody+(12)/pmc11392954-133-60-65
    Average 95 stars, based on 46 article reviews
    rat antibody against mbp - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Labeling:

    Article Title: Microglial process convergence onto injured axonal swellings, a human postmortem brain tissue study
    Article Snippet: Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the next secondary antibody incubation with Alexa Fluor 647-conjugated goat anti-mouse IgG (1:700; Cat.# A- 21237, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2hr. .. Iba-1 and APP labeled tissue was washed in PBS at least four times prior to overnight incubation with a rat antibody against MBP (1:200; Novus) at 4C° in 5% NGS/2% BSA/0.5% triton. .. Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the third secondary antibody incubation with Alexa Fluor 568-conjugated goat anti-rat IgG (1:700; Cat.# A- 11077, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2hr.

    Article Title: Microglial process convergence onto injured axonal swellings, a human postmortem brain tissue study
    Article Snippet: Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to secondary antibody incubation with Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:700; Cat.# A-11008, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2 h. Tissue was washed in PBS at least four times prior to overnight incubation with a mouse antibody against the 22C11 clone of APP (1:200; Cat.#14-9749-82, ThermoFisher Scientific, Waltham, MA, USA) in 5% NGS/2% BSA/0.5% triton at 4C°. .. Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the next secondary antibody incubation with Alexa Fluor 647-conjugated goat anti-mouse IgG (1:700; Cat.# A-21237, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2 h. Iba-1 and APP labeled tissue was washed in PBS at least four times prior to overnight incubation with a rat antibody against MBP (1:200; Novus) at 4C° in 5% NGS/2% BSA/0.5% triton. .. Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the third secondary antibody incubation with Alexa Fluor 568-conjugated goat anti-rat IgG (1:700; Cat.# A-11077, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2 h. Tissue was washed in PBS at least four times.

    Incubation:

    Article Title: Microglial process convergence onto injured axonal swellings, a human postmortem brain tissue study
    Article Snippet: Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the next secondary antibody incubation with Alexa Fluor 647-conjugated goat anti-mouse IgG (1:700; Cat.# A- 21237, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2hr. .. Iba-1 and APP labeled tissue was washed in PBS at least four times prior to overnight incubation with a rat antibody against MBP (1:200; Novus) at 4C° in 5% NGS/2% BSA/0.5% triton. .. Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the third secondary antibody incubation with Alexa Fluor 568-conjugated goat anti-rat IgG (1:700; Cat.# A- 11077, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2hr.

    Article Title: Microglial process convergence onto injured axonal swellings, a human postmortem brain tissue study
    Article Snippet: Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to secondary antibody incubation with Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:700; Cat.# A-11008, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2 h. Tissue was washed in PBS at least four times prior to overnight incubation with a mouse antibody against the 22C11 clone of APP (1:200; Cat.#14-9749-82, ThermoFisher Scientific, Waltham, MA, USA) in 5% NGS/2% BSA/0.5% triton at 4C°. .. Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the next secondary antibody incubation with Alexa Fluor 647-conjugated goat anti-mouse IgG (1:700; Cat.# A-21237, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2 h. Iba-1 and APP labeled tissue was washed in PBS at least four times prior to overnight incubation with a rat antibody against MBP (1:200; Novus) at 4C° in 5% NGS/2% BSA/0.5% triton. .. Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the third secondary antibody incubation with Alexa Fluor 568-conjugated goat anti-rat IgG (1:700; Cat.# A-11077, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2 h. Tissue was washed in PBS at least four times.

    Next-Generation Sequencing:

    Article Title: Microglial process convergence onto injured axonal swellings, a human postmortem brain tissue study
    Article Snippet: Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the next secondary antibody incubation with Alexa Fluor 647-conjugated goat anti-mouse IgG (1:700; Cat.# A- 21237, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2hr. .. Iba-1 and APP labeled tissue was washed in PBS at least four times prior to overnight incubation with a rat antibody against MBP (1:200; Novus) at 4C° in 5% NGS/2% BSA/0.5% triton. .. Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the third secondary antibody incubation with Alexa Fluor 568-conjugated goat anti-rat IgG (1:700; Cat.# A- 11077, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2hr.

    Article Title: Microglial process convergence onto injured axonal swellings, a human postmortem brain tissue study
    Article Snippet: Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to secondary antibody incubation with Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:700; Cat.# A-11008, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2 h. Tissue was washed in PBS at least four times prior to overnight incubation with a mouse antibody against the 22C11 clone of APP (1:200; Cat.#14-9749-82, ThermoFisher Scientific, Waltham, MA, USA) in 5% NGS/2% BSA/0.5% triton at 4C°. .. Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the next secondary antibody incubation with Alexa Fluor 647-conjugated goat anti-mouse IgG (1:700; Cat.# A-21237, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2 h. Iba-1 and APP labeled tissue was washed in PBS at least four times prior to overnight incubation with a rat antibody against MBP (1:200; Novus) at 4C° in 5% NGS/2% BSA/0.5% triton. .. Tissue was washed with 1%NGS/1%BSA in PBS at least six times prior to the third secondary antibody incubation with Alexa Fluor 568-conjugated goat anti-rat IgG (1:700; Cat.# A-11077, Life Technologies, Carlsbad, CA, USA) in 1%NGS/1%BSA/PBS at room temperature for 2 h. Tissue was washed in PBS at least four times.



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    Image Search Results


    Localization of CD59 in murine teased nerve and myelinated cultures. A CD59 (green) and ankyrin-G (AnkG, red) staining of WT teased fibers. B CD59 (green) and Caspr (red) staining of WT teased fibers. C CD59 (green) and neurofascin (Nfasc, blue) staining of WT teased fibers. D CD59 (green), myelin basic protein (MBP, red), and neurofascin (Nfasc, blue) staining of WT ICR DRG. CD59 is co-localized with MBP and localized in the internodes and paranodes. There is no CD59 localization in the nodes of Ranvier. A – C Sections taken from a 4-month-old mouse. Immunolabeling procedure handled with methanol and 0.1% triton. Scale bars 20 μm and in D 50 μm

    Journal: Journal of Neuroinflammation

    Article Title: Complement-membrane regulatory proteins are absent from the nodes of Ranvier in the peripheral nervous system

    doi: 10.1186/s12974-023-02920-9

    Figure Lengend Snippet: Localization of CD59 in murine teased nerve and myelinated cultures. A CD59 (green) and ankyrin-G (AnkG, red) staining of WT teased fibers. B CD59 (green) and Caspr (red) staining of WT teased fibers. C CD59 (green) and neurofascin (Nfasc, blue) staining of WT teased fibers. D CD59 (green), myelin basic protein (MBP, red), and neurofascin (Nfasc, blue) staining of WT ICR DRG. CD59 is co-localized with MBP and localized in the internodes and paranodes. There is no CD59 localization in the nodes of Ranvier. A – C Sections taken from a 4-month-old mouse. Immunolabeling procedure handled with methanol and 0.1% triton. Scale bars 20 μm and in D 50 μm

    Article Snippet: For murine sciatic nerve and teased cross- and longitudinal sections (including those prepared with the TSA method), we used rabbit polyclonal antibody (pAb) against mouse CD59 (Cardiff University), rat monoclonal antibody (mAb) against mouse CD59 (Cardiff University), chicken pAb against mouse myelin protein zero (P0, AVES-2B Scientific, Oxfordshire, UK), mouse mAb against mouse ankyrinG (AnkG, NeuroMab, Davis, CA, USA), rat mAb against mouse myelin basic protein (MBP) (Millipore, Burlington, MA, USA), rabbit mAb against mouse Caspr (Weizmann Institute [ ]), rat mAb against mouse DAF/CD55 (Cardiff University, 2C6), rabbit pAb against mouse Crry (Biorbyt, Cambridge, UK), rabbit pAb against mouse CD46 (Abcam, Cambridge, UK), mouse mAb against mouse beta dystroglycan (Novocastra, Newcastle on Tyre, UK), mouse mAb against mouse myelin-associated glycoprotein (MAG) (Chemicon International, Temecula, CA, USA), chicken pAb against mouse neurofascin (Nfasc, R&D, Minneapolis, MN, USA), rabbit pAb against mouse CD31 (Abcam, Cambridge, UK), and rat mAb against mouse CD31 (Abcam, Cambridge, UK).

    Techniques: Staining, Immunolabeling